During last decade, the research carried out in our laboratory has been centered in studying the mechanisms of action of Aβ and seeking to elucidate how the peptide can perturb the excitatory-inhibitory balance (E/I ratio) of neural circuitries in the brain; a neurotransmission imbalance which, in turn, results in altered synaptic plasticity and network activity, presumably leading to cognitive decline and learning and memory deficits in AD. Our long-term goal is to make a progress in the understanding of the mechanisms underlying AD at molecular, cellular, synaptic, neuronal network and behavioral levels, in order to search efficient strategies to prevent and treat this dementia.
In our studies we use different
experimental approaches including:
- Extra and intracellular (patch- clamp technics) recordings of neuronal activity ex vivo.
- Multielectrode Array extracelullar recordings.
- Electroencephalography (EEG) and local field potentials (LFP) recordings to assess neuron activity in vivo in behaving animals.
- Pharmacological manipulations to manipulate specific pathways in vivo.
- Electrophysiology and behavioral tests combined in freely moving animals to determine the cognitive consequences of our mechanistic interventions.